Anti-Histone H2B (crotonyl K20) antibody (ab240892)
Key features and details
- Rabbit polyclonal to Histone H2B (crotonyl K20)
- Suitable for: WB, ICC/IF, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2B (crotonyl K20) antibody
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (crotonyl K20) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Histone H2B (crotonyl K20). The exact sequence is proprietary.
Database link: P62807 -
Positive control
- WB: K562 and Jurkat, treated with with 30mM sodium crotonylate for 4hr, whole cell lysates. ICC/IF: HeLa cells (treated with 30mM sodium crotonylate for 4hr). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium crotonylate for 4hr).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H2B (crotonyl K20) antibody (ab240892)All lanes : Anti-Histone H2B (crotonyl K20) antibody (ab240892) at 1/100 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium crotonylate for 4hr
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate, treated (+) with 30mM sodium crotonylate for 4hr
Lane 3 : K562 whole cell lysate, untreated (-)
Lane 4 : Jurkat whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (crotonyl K20) antibody (ab240892)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30mM sodium butyrate for 4hr) stained for Histone H2B (crotonyl K20) using ab240892 at 1/12.5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was an Alexa-Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).
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ChIP - Anti-Histone H2B (crotonyl K20) antibody (ab240892)HeLa (human epithelial cell line from cervix adenocarcinoma; 106, treated with 30mM sodium crotonylatefor 4hr) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240892 or a control normal rabbit IgG.
The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
