Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Thursday March 04, 2021

Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15)
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Histone H2B (acetyl K15) antibody [EP955Y]
See all Histone H2B primary antibodies
Description

Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15)
Host species

Rabbit
Specificity

This antibody only detects Histone H2B acetylated on Lysine 15.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K15). The exact sequence is proprietary.
Positive control
- HeLa whole cell lysate (ab150035) or human breast carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP955Y
Isotype

IgG
Research areas

Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)

All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution

Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with TSA)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

The bottom image shows beta Tubulin as a positive control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)

Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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