Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [IGX4228R-1] to Histone H2B
Suitable for: WB, ChIP
Reacts with: Mouse, Cow, Human, Recombinant fragment

Product name

Anti-Histone H2B antibody [IGX4228R-1]
See all Histone H2B primary antibodies
Description

Rabbit monoclonal [IGX4228R-1] to Histone H2B
Host species

Rabbit
Specificity

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Tested Applications & Species

Application	Species
ChIP	Mouse Human
WB	Mouse Cow Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Histone H2B.
Positive control
- WB: CTH, HeLa whole cell, HeLa Nuclear, NIH3T3 whole cell, NIH3T3 Nuclear and Histone H2B Recombinant Protein lysates. ChIP: HeLa and NIH3T3.
General notes

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

For recommended secondary antibodies -

Goat Anti-Rabbit IgG H&L (HRP) - ab205718

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) - ab150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) - ab150078

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) - ab150079

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) - ab150080
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

IGX4228R-1
Isotype

IgG1

Western blot - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

All lanes : Anti-Histone H2B antibody [IGX4228R-1] (ab213225) at 0.25 µg/ml

Lane 1 : CTH (Calf Thymus Histone) at 0.5 µg
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg
Lane 6 : Histone H2B Recombinant Protein at 0.1 µg
Lane 7 : Histone H3 Recombinant Protein at 0.1 µg

Secondary
All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 14 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213225 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ChIP - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213225 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
ChIP - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213225 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

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