Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)
Key features and details
- Rabbit polyclonal to Histone H2B (acetyl K15) - ChIP Grade, purified
- Suitable for: ICC/IF, Dot blot, ChIP-sequencing, WB, ChIP
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (acetyl K15) - ChIP Grade, purified -
Host species
Rabbit -
Specificity
Due to sequence homology we expect this antibody to detect K15 acetylation on multiple H2B variants. -
Tested applications
Suitable for: ICC/IF, Dot blot, ChIP-sequencing, WB, ChIPmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human Histone H2B (acetyl K15) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. (The region of Histone H2B containing the acetylated lysine 15 (H2BK15ac)).
Database link: Q5QNW6 -
Positive control
- Whole cell and histone extracts from Hela cells; chromatin from Hela cells and HeLaS3 cells; recombinant histone H2A, H2B, H3 and H4. HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)
ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 2 µg of ab195652, as described for the CHIP application. The IP’d DNA was subsequently analysed on a sequencer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the Human genome using the BWA algorithm. This image shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (sections A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the GAPDH gene (sections C and D). The position of the amplicon used for ChIPqPCR is indicated by an arrow.
-
ChIP - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)CHIP analysis of Histone H2B type 1-C/E/F/G/I (acetyl K15) in HeLa cells using ab195652 at 2 μg/IP, and optimized PCR primer sets for qPCR. ChIP was performed with the kit on sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 µg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
-
Dot Blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)To test the cross reactivity of ab195652, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified Histone H2B type 1-C/E/F/G/I. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1/20,000. This image shows a high specificity of the antibody for the modification of interest.
-
Western blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)All lanes : Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652) at 1/500 dilution
Lane 1 : whole extract from Hela cell at 25 µg
Lane 2 : histone extract from Hela cell at 15 µg
Lane 3 : recombinant histone H2A at 1 µg
Lane 4 : recombinant histone H2B at 1 µg
Lane 5 : recombinant histone H3 at 1 µg
Lane 6 : recombinant histone H4 at 1 µg
Predicted band size: 14 kDaab195652 was diluted in TBS-Tween containing 5% skimmed milk.
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)Immunofluorescent analysis of Histone H2B type 1-C/E/F/G/I (acetyl K15) in HeLa cells using ab195652 at a 1/500 dilution in blocking solution, followed by an anti-rabbit antibody conjugated to Alexa488 (left). The cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
