Anti-Histone H3 (phospho S10) antibody (ab47297)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho S10)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho S10) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho S10) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Histone H3.1 only when phosphorylated at Serine 10. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized phosphopeptide derived from human Histone H3 around the phosphorylation site of serine 10 (R-K-SP-T-G)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab47297)All lanes : Anti-Histone H3 (phospho S10) antibody (ab47297)
Lane 1 : EGF + Calyculin treated HeLa cells + phospho-peptide.
Lane 2 : EGF + Calyculin treated HeLa cells. No phosphopeptide.
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (ab47297)ab47297 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of immunizing phosphopeptide.
