Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y28] to Histone H3 (acetyl K9) - BSA and Azide free
  • Suitable for: ChIP-sequencing, IP, Flow Cyt, IHC-P, ICC/IF, ChIP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [Y28] to Histone H3 (acetyl K9) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ChIP-seq
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NIH 3T3 cell lysate and human lung carcinoma IHC-P: Human normal colon FFPE tissue sections. ChIP: Chromatin prepared from HeLa cells. ChIP-seq: Chromatin prepared from HeLa cells. IP: HeLa cells. ICC/IF: HeLa cells.

  • General notes

    Ab203951 is the carrier-free version of ab32129. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab203951 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    This data was developed using the same antibody clone in a different buffer formulation (ab32129).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 µg of Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129). ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

    Additional screenshots of mapped reads can be downloaded here.

  • ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    This data was developed using the same antibody clone in a different buffer formulation (ab32129).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129). ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.

    Additional screenshots of mapped reads can be downloaded here.

  • ChIP - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    ChIP - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 2 µg of ab32129 (red), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
    Primers and probes are located in the first kb of the transcribed region.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32129).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Histone H3 with purified ab32129 at 1/250 dilution (4 µg/mL). Cells were fixed in 100% Methano. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32129).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    IHC image of ab32129 staining Histone H3 (acetyl K9) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32129, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32129).

  • Flow Cytometry - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    Flow Cytometry - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab32129 at 1/230 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32129).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32129 at 1/50 - 1/100

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32129).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)
    Anti-Histone H3 (acetyl K9) antibody [Y28] - BSA and Azide free (ab203951)

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