Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 405 Rabbit monoclonal [EPR3776] to Vimentin - Cytoskeleton Marker
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Overview

  • Product name

    Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
    See all Vimentin primary antibodies

  • Description

    Alexa Fluor® 405 Rabbit monoclonal [EPR3776] to Vimentin - Cytoskeleton Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rhesus monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa cells. Wildtype HAP-1 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)
    Flow Cytometry - Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)
    Overlay histogram showing HAP1 wildtype (green line) and HAP1-VIM knockout cells (red line) stained with ab210152. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210152, 0.1µg/1x106 cells OR 1/500 dilution) for 30 min at 22°C.
    A rabbit IgG isotype control antibody  (ab208150) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-VIM knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
    Acquisition of >5,000 events were collected using a 50 mW Violet laser (405nm) and 450/50 bandpass filter.
    This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
  • Flow Cytometry - Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)
    Flow Cytometry - Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)

    Overlay histogram showing HeLa cells stained with ab210152 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210152, 1/500 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.

  • Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)
    Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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