Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)
![Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)](https://www.abcam.com/ps/products/247/ab247569/Images/ab247569-403261-anti-brg1-antibody-epr3913-bsa-and-azide-free-western-blot.jpg "Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3913] to BRG1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-BRG1 antibody [EPR3913] - BSA and Azide free
See all BRG1 primary antibodies -
Description
Rabbit monoclonal [EPR3913] to BRG1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247569 is the carrier-free version of ab92496 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247569 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3913 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)
This data was developed using ab92496, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92496 observed at 185 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab92496 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab92496 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)](https://www.abcam.com/ps/products/247/ab247569/Images/ab247569-397996-anti-brg1-antibody-epr3913-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)All lanes : Anti-BRG1 antibody [EPR3913] (ab92496) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 185 kDaThis data was developed using ab92496, the same antibody clone in a different buffer formulation.
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![Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)](https://www.abcam.com/ps/products/247/ab247569/Images/ab247569-3-benefits-of-recombinant-antibodies.png)
Anti-BRG1 antibody [EPR3913] - BSA and Azide free (ab247569)
