Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15241] to PGM1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PGM1 antibody [EPR15241] - BSA and Azide free
    See all PGM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15241] to PGM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251158 is the carrier-free version of ab192876. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    All lanes : Anti-PGM1 antibody [EPR15241] (ab192876) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    This data was developed using ab192876, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    Immunocytochemistry - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    This data was developed using ab192876, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling PGM1 with ab192876 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stain is DAPI (blue).
  • Flow Cytometry - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    Flow Cytometry - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    This data was developed using ab192876, the same antibody clone in a different buffer formulation.ab192876 staining PGM1 in A549 (human lung carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/90. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. Isoytype control: Rabbit monoclonal IgG (Black) Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
  • Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    All lanes : Anti-PGM1 antibody [EPR15241] (ab192876) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat kidney tissue lysate
    Lane 6 : Rat spleen tissue lysate
    Lane 7 : C6 cell lysate
    Lane 8 : RAW 264.7 cell lysate
    Lane 9 : PC12 cell lysate
    Lane 10 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    This data was developed using ab192876, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    Immunoprecipitation - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    This data was developed using ab192876, the same antibody clone in a different buffer formulation.Western blot analysis of PGM1 immunoprecipitated from Human fetal liver lysate with ab192876 at 1/60 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.
  • Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)
    Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

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