Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17737] to AKT1 + AKT2 + AKT3 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free
    See all AKT1 + AKT2 + AKT3 primary antibodies

  • Description

    Rabbit monoclonal [EPR17737] to AKT1 + AKT2 + AKT3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251298 is the carrier-free version of ab200195. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251298 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Immunocytochemistry - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)

    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AKT1 + AKT2 + AKT3 with ab200195 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200195 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution

    Lane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479
    Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481
    Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa


    Exposure time: 3 seconds


    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    AKT3 recombinant protein fragment contains aa351-479 with His-Tag®. AKT2 recombinant protein fragment contains aa282-481 with His-Tag® and AKT1 recombinant protein fragment contains aa281-480 with His-Tag®.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 15 seconds


    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 3 minutes


    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/10000 dilution

    Lane 1 : PC-3 (Human prostate cancer cell line) whole cell lysate
    Lane 3 : 293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 30 seconds


    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 15 seconds


    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 10 seconds


    This data was developed using ab200195, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)
    Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] - BSA and Azide free (ab251298)

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