Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free (ab247377)
![Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free (ab247377)](https://www.abcam.com/ps/products/247/ab247377/Images/ab247377-397913-anti-braf-phospho-t401-antibody-epr2208y-bsa-and-azide-free-western-blot.jpg "Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free (ab247377)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2208Y] to BRAF (phospho T401) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free
See all BRAF primary antibodies -
Description
Rabbit monoclonal [EPR2208Y] to BRAF (phospho T401) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects B Raf phosphorylated on threonine 401.
-
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab247377 is the carrier-free version of ab68215. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247377 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2208Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free (ab247377)All lanes : Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilution
Lane 1 : PC-12 cell lysates (cells were untreated)
Lane 2 : PC-12 cell lysates (cells were treated with TPA)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDaThis data was developed using ab68215, the same antibody clone in a different buffer formulation.
-
![Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free (ab247377)](https://www.abcam.com/ps/products/247/ab247377/Images/ab247377-2-benefits-of-recombinant-antibodies.png)
Anti-BRAF (phospho T401) antibody [EPR2208Y] - BSA and Azide free (ab247377)
