Recombinant human ALK (mutated F1174L) protein (ab186454)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human ALK (mutated F1174L) protein
See all ALK proteins and peptides -
Biological activity
The specific activity of ab186454 was determined to be 112 nmol /min/mg as per activity assay protocol.
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Purity
> 75 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RRKHQELQAMQ MELQSPEYKL SKLRTSTIMT DYNPNYCFAG KTSSISDLKE VPRKNITLIR GLGHGAFGEV YEGQVSGMPN DPSPLQVAVK TLPEVCSEQD ELDFLMEALI ISKLNHQNIV RCIGVSLQSL PRFILLELMA GGDLKSFLRE TRPRPSQPSS LAMLDLLHVA RDIACGCQYL EENHFIHRDI AARNCLLTCP GPGRVAKIGD FGMARDIYRA SYYRKGGCAM LPVKWMPPEA FMEGIFTSKT DTWSFGVLLW EIFSLGYMPY PSKSNQEVLE FVTSGGRMDP PKNCPGPVYR IMTQCWQHQP EDRPNFAIIL ERIEYCTQDP DVINTALPIE YGPLVEEEEK VPVRPKDPEG VPPLLVSQQA KREEERSPAA PPPLPTTSSG KAAKKPTAAE ISVRVPRGPA VEGGHVNMAF SQSNPPSELH KVHGSRNKPT SLWNPTYGSW FTEKPTKKNN PIAKKEPHDR GNLGLEGSCT VPPNVATGRL PGASLLLEPS SLTANMKEVP LFRLRHFPCG NVNYGYQQQG LPLEAATAPG AGHYEDTILK SKNSMNQPGP -
Predicted molecular weight
90 kDa including tags -
Amino acids
1060 to 1620 -
Modifications
mutated F1174L -
Tags
proprietary tag N-Terminus -
Additional sequence information
NM_004304.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Preservative: 1.02% Imidazole
Constituents: 0.71% Sodium phosphate, 1.75% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human ALK (mutated F1174L) protein (ab186454)
SDS-PAGE analysis of ab186454.
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Functional Studies - Recombinant human ALK (mutated F1174L) protein (ab186454)Kinase Assay demonstrating specific activity of ab186454.
