Recombinant human ALK (mutated R1275Q) protein (ab186455)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human ALK (mutated R1275Q) protein
See all ALK proteins and peptides -
Biological activity
The specific activity of ab186455 was determined to be 19 nmol /min/mg as per activity assay protocol.
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Purity
> 90 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLK EVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQ DELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLR ETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTC PGPGRVAKIGDFGMAQDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSK TDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVY RIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEE KVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAA EISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGS WFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEP SSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTIL KSKNSMNQPGP -
Predicted molecular weight
90 kDa -
Amino acids
1060 to 1620 -
Modifications
mutated R1275Q -
Tags
proprietary tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Preservative: 1.02% Imidazole
Constituents: 0.81% Sodium phosphate, 1.75% Sodium chloride, 0.002% PMSF, 0.005% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human ALK (mutated R1275Q) protein (ab186455)The specific activity of ALK (ab186455) was determined to be 10 nmol/min/mg as per activity assay protocol and was equivalent to 21 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human ALK (mutated R1275Q) protein (ab186455)SDS PAGE analysis of ab186455 -
SDS-PAGE - Recombinant human ALK (mutated R1275Q) protein (ab186455)SDS PAGE analysis of ab186455 -
SDS-PAGE - Recombinant human ALK (mutated R1275Q) protein (ab186455)SDS-PAGE analysis of ab186455.
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Functional Studies - Recombinant human ALK (mutated R1275Q) protein (ab186455)Kinase Assay demonstrating the specific activity of ab186455.
