Anti-OPA1 antibody [1E81D9] (ab119685)
![Anti-OPA1 antibody [1E81D9] (ab119685)](https://www.abcam.com/ps/products/119/ab119685/Images/ab119685-204494-anti-opa1-antibody-1e81d9-flow-cytometry.jpg "Anti-OPA1 antibody [1E81D9] (ab119685)")
Key features and details
- Mouse monoclonal [1E81D9] to OPA1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-OPA1 antibody [1E81D9]
See all OPA1 primary antibodies -
Description
Mouse monoclonal [1E81D9] to OPA1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab119685 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
1E81D9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)](https://www.abcam.com/ps/products/119/ab119685/Images/ab119685-132834-anti-opa1-antibody-1e81d9-western-blot.jpg)
Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml
Lane 1 : whole cell lysates from HeLa cells(human)
Lane 2 : whole cell lysates from H4IIE cells(rat)
Lane 3 : whole cell lysates from MEF cells(mouse)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP goat anti-mouse at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
