Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11057(B)] to OPA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free
    See all OPA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11057(B)] to OPA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A431 cell lysates; IHC-P: Human brain and retina tissue; IHC-Fr: Mouse retina tissue.

  • General notes

    ab240143 is the carrier-free version of ab157457.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

    Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

    Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

    Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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