Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-OPA1 antibody [EPR11057(B)] (ab157457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11057(B)] to OPA1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-OPA1 antibody [EPR11057(B)]
    See all OPA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11057(B)] to OPA1

  • Host species

    Rabbit

  • Specificity

    Recent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human OPA1 aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: O60313

  • Positive control

    • WB: HeLa, HepG2, A431 cell lysates; IHC-P: Human brain and retina tissue; IHC-Fr: Mouse retina tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)

    Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 112 kDa
    Observed band size: 85-100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)

    Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    All lanes : Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 112 kDa

  • Anti-OPA1 antibody [EPR11057(B)] (ab157457)
    Anti-OPA1 antibody [EPR11057(B)] (ab157457)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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