Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Neurogranin antibody [NG22] (ab273444)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [NG22] to Neurogranin
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Neurogranin antibody [NG22]
See all Neurogranin primary antibodies
Description

Mouse monoclonal [NG22] to Neurogranin
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain lysate. IHC-P: Human cerebrum tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

NG22
Isotype

IgG1
Research areas

Western blot - Anti-Neurogranin antibody [NG22] (ab273444)

All lanes : Anti-Neurogranin antibody [NG22] (ab273444) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : Human lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 8 kDa

Exposure time: 37 seconds

Negative control: Human lung (PMID: 20665622).
The molecular weight observed is consistent with what has been described in the literature (PMID: 20875798).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody (ab273444)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human cererbum. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody (ab273444)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control: No staining on human lung. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Anti-Neurogranin antibody [NG22] (ab273444)

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