Anti-AK2 antibody (ab228786)
Key features and details
- Rabbit polyclonal to AK2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AK2 antibody
See all AK2 primary antibodies -
Description
Rabbit polyclonal to AK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human AK2 (internal sequence). The exact sequence is proprietary.
Database link: P54819 -
Positive control
- WB: NCI-H1299 and HepG2 whole cell lysates. IHC-P: Rat liver tissue; NCIN87 xenograft tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AK2 antibody (ab228786)All lanes : Anti-AK2 antibody (ab228786) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : AK2 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa
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Western blot - Anti-AK2 antibody (ab228786)All lanes : Anti-AK2 antibody (ab228786) at 1/1000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody (ab228786)Paraffin-embedded rat liver tissue stained for AK2 using ab228786 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody (ab228786)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) stained for AK2 (green) using ab228786 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody (ab228786)Paraffin-embedded NCIN87 xenograft stained for AK2 using ab228786 at 1/500 dilution in immunohistochemical analysis.
