Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-AK2 antibody [EPR11387(B)] (ab157206)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11387(B)] to AK2
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-AK2 antibody [EPR11387(B)]
See all AK2 primary antibodies
Description

Rabbit monoclonal [EPR11387(B)] to AK2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human AK2 (Uniprot: P54819)
Positive control
- WB: HEK-293, HepG2 and HL-60 cell lysates; Fetal kidney, heart and liver lysates; Recombinant human AK2 protein. IHC-P: Human heart and liver tissues. Flow Cyt: HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11387(B)
Isotype

IgG
Research areas

Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)

All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : AK2 knockout HEK293T cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 26 kDa
Observed band size: 30 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab157206 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

ab157206 Anti-AK2 antibody [EPR11387(B)] was shown to specifically react with AK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266539 (knockout cell lysate ab257825) was used. Wild-type and AK2 knockout samples were subjected to SDS-PAGE. ab157206 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling AK2 using ab157206 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-AK2 antibody [EPR11387(B)] (ab157206)

Flow cytometry analysis of permeabilized HeLa cells labeling AK2 (red), using ab157206 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling AK2 using ab157206 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)

All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution

Lane 1 : HepG2 lysate
Lane 2 : fetal kidney lysate
Lane 3 : fetal heart lysate
Lane 4 : HeLa lysate
Lane 5 : fetal liver lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 26 kDa
Anti-AK2 antibody [EPR11387(B)] (ab157206)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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