Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AK2 antibody [EPR11387(B)] (ab157206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11387(B)] to AK2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-AK2 antibody [EPR11387(B)]
    See all AK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11387(B)] to AK2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human AK2 (Uniprot: P54819)

  • Positive control

    • WB: HEK-293, HepG2 and HL-60 cell lysates; Fetal kidney, heart and liver lysates; Recombinant human AK2 protein. IHC-P: Human heart and liver tissues. Flow Cyt: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : AK2 knockout HEK293T cell lysate
    Lane 3 : HL-60 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab157206 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab157206 Anti-AK2 antibody [EPR11387(B)] was shown to specifically react with AK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266539 (knockout cell lysate ab257825) was used. Wild-type and AK2 knockout samples were subjected to SDS-PAGE. ab157206 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling AK2 using ab157206 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Flow Cytometry - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Flow cytometry analysis of permeabilized HeLa cells labeling AK2 (red), using ab157206 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling AK2 using ab157206 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)
    All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : fetal kidney lysate
    Lane 3 : fetal heart lysate
    Lane 4 : HeLa lysate
    Lane 5 : fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • Anti-AK2 antibody [EPR11387(B)] (ab157206)
    Anti-AK2 antibody [EPR11387(B)] (ab157206)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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