Anti-ROCK2 (phospho S1366) antibody (ab228008)
Key features and details
- Rabbit polyclonal to ROCK2 (phospho S1366)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ROCK2 (phospho S1366) antibody
See all ROCK2 primary antibodies -
Description
Rabbit polyclonal to ROCK2 (phospho S1366) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Rhesus monkey -
Immunogen
Synthetic peptide within Human ROCK2 (C terminal) (phospho S1366). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: O75116 -
Positive control
- WB: HEK-293T and HeLa whole cell extracts; HEK-293T transfected with WT ROCK2 whole cell lysate. IHC-P: Human breast tissue; Mouse brain tissue; Rat kidney tissue; HBL435 xenograft tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : ROCK2 shRNA transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 161 kDa5% SDS-PAGE gel.
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All lanes : Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with CIP (calf-intestinal alkaline phosphatase)
Lysates/proteins at 30 µg per lane.
Predicted band size: 161 kDa5% SDS-PAGE gel.
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All lanes : Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/1000 dilution
Lanes 1 & 3 & 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with WT ROCK2, whole cell lysate
Lanes 2 & 4 & 6 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with phospho-mutant (S1366A) ROCK2, whole cell lysate
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 161 kDaHEK-293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCK2 expression constructs. Overexpressed Myc-ROCK2 proteins were immunoprecipitated with anti-myc antibody and probed with ab228008 with or without peptide (0.2 mg/ml) competition as indicated.
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Paraffin-embedded HBL435 xenograft tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human breast tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat kidney tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
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Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROCK2 (phospho S1366) (green) using ab228008 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).