Anti-SPR antibody [EPR9290] (ab157194)
![Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-384453-anti-spr-antibody-epr9290-western-blot-hela-mcf7.jpg "Anti-SPR antibody [EPR9290] (ab157194)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9290] to SPR
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SPR antibody [EPR9290]
See all SPR primary antibodies -
Description
Rabbit monoclonal [EPR9290] to SPR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to residues in Human SPR (UniProt: P35270).
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Positive control
- WB: A549, MCF7, HepG2 and HeLa (ab150035) cell lysates. IHC-P: Human thyroid gland carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: MCF7 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9290 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPR antibody [EPR9290] (ab157194)All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SPR knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab157194 observed at 26 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab157194 Anti-SPR antibody [EPR9290] was shown to specifically react with SPR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264681 (knockout cell lysate ab258700) was used. Wild-type and SPR knockout samples were subjected to SDS-PAGE. ab157194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-164356-anti-spr-antibody-epr9290-western-blot.jpg)
Western blot - Anti-SPR antibody [EPR9290] (ab157194)All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-168885-anti-spr-antibody-epr9290-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)ab157194 showing +ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-168884-anti-spr-antibody-epr9290-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)ab157194 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-168883-anti-spr-antibody-epr9290-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)ab157194 showing +ve staining in Human endometrial carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-168882-anti-spr-antibody-epr9290-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)ab157194 showing +ve staining in Human colonic adenocarcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-164355-anti-spr-antibody-epr9290-flow-cytometry.jpg)
Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)Flow cytometic analysis of permeabilized MCF7 cells labeling TPST2 with ab157194 at 1/100 (red) or a rabbit IgG (negative) (green). -
![Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-164354-anti-spr-antibody-epr9290-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)Immunofluorescent analysis of HeLa cells labeling TPST2 with ab157194 at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-164353-anti-spr-antibody-epr9290-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)Immunohistochemical analysis of paraffin-eEmbedded Human thyroid gland carcinoma tissue labeling TPST2 with ab157194 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-164352-anti-spr-antibody-epr9290-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)Detection of TPST2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab157194 at 1/10 dilution. -
![Anti-SPR antibody [EPR9290] (ab157194)](https://www.abcam.com/ps/products/157/ab157194/Images/ab157194-11-benefits-of-recombinant-antibodies.png)
Anti-SPR antibody [EPR9290] (ab157194)
