Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SPR antibody [EPR9290] (ab157194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9290] to SPR
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SPR antibody [EPR9290]
    See all SPR primary antibodies

  • Description

    Rabbit monoclonal [EPR9290] to SPR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to residues in Human SPR (UniProt: P35270).

  • Positive control

    • WB: A549, MCF7, HepG2 and HeLa (ab150035) cell lysates. IHC-P: Human thyroid gland carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: MCF7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SPR antibody [EPR9290] (ab157194)
    Western blot - Anti-SPR antibody [EPR9290] (ab157194)
    All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SPR knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab157194 observed at 26 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab157194 Anti-SPR antibody [EPR9290] was shown to specifically react with SPR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264681 (knockout cell lysate ab258700) was used. Wild-type and SPR knockout samples were subjected to SDS-PAGE. ab157194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-SPR antibody [EPR9290] (ab157194)
    Western blot - Anti-SPR antibody [EPR9290] (ab157194)
    All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

    ab157194 showing +ve staining in Human normal pancreas.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

    ab157194 showing +ve staining in Human heart.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

    ab157194 showing +ve staining in Human endometrial carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

    ab157194 showing +ve staining in Human colonic adenocarcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)
    Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)
    Flow cytometic analysis of permeabilized MCF7 cells labeling TPST2 with ab157194 at 1/100 (red) or a rabbit IgG (negative) (green).
  • Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)
    Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)
    Immunofluorescent analysis of HeLa cells labeling TPST2 with ab157194 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

    Immunohistochemical analysis of paraffin-eEmbedded Human thyroid gland carcinoma tissue labeling TPST2 with ab157194 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)
    Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)
    Detection of TPST2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab157194 at 1/10 dilution.
  • Anti-SPR antibody [EPR9290] (ab157194)
    Anti-SPR antibody [EPR9290] (ab157194)

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