Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYSM1 antibody [EPR18657] (ab193081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18657] to MYSM1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYSM1 antibody [EPR18657]
    See all MYSM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18657] to MYSM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, HepG2, NCCIT, K562, RAW 264.7, PC-12, NIH/3T3 and mESC whole cell lysates. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 5 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 66,95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Two bands detected represent two isoforms.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution + mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)

    MYSM1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab193081 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab193081 IP in HeLa whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193081 in HeLa whole cell lysate.

    Predicted band size: 66kDa, 95kDa.

    Observed band size: 66kDa, 95kDa.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Two bands detected represent two isoforms.

  • Anti-MYSM1 antibody [EPR18657] (ab193081)
    Anti-MYSM1 antibody [EPR18657] (ab193081)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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