Anti-Ube4a antibody [EPR7331] (ab128861)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7331] to Ube4a
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Ube4a antibody [EPR7331]
See all Ube4a primary antibodies -
Description
Rabbit monoclonal [EPR7331] to Ube4a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
A synthetic peptide corresponding to residues in Human Ube4a (UniProt Q14139).
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Positive control
- 293T, K562, Caco 2 and HepG2 cell lysates; Caco 2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.91 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7331 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 123 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
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ab128861, at 1/100 dilution, staining Ube4a in Caco 2 cells by Immunofluorescence.
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