Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free
See all UBE2C primary antibodies
Description

Rabbit monoclonal [EPR23165-31] to UBE2C - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: T-47D, SW480, NIH:OVCAR-3, HeLa, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and rat spleen tissue lysate. IHC-P: Human gastric cancer tissue and colon tissue. Mouse and rat colon tissue. ICC/IF: SW480 and RAW 264.7 cells. IP: SW480 and NIH:OVCAR-3 whole cell lysate.
General notes
ab269592 is the carrier-free version of ab252940.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23165-31
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling UBE2C with ab252940 at 1/50 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in partial RAW 264.7 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling UBE2C with ab252940 at 1/50 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in partial SW480 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in proliferative cells of rat colon is observed (PMID: 15208666). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in proliferative cells of mouse colon is observed (PMID: 15208666). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling UBE2C with ab252940 at 1/500

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasm and nuclear staining in proliferative cells of human gastric cancer is observed (PMID:15208666, 27349176). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasm and nuclear staining in proliferative cells of human colon is observed (PMID: 15208666). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunoprecipitation - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

UBE2C was immunoprecipitated from 0.35 mg NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate 10µg with ab252940 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252940 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: NIH:OVCAR-3 whole cell lysate 10µg.

Lane 2: ab252940 IP in NIH:OVCAR-3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252940 in NIH:OVCAR-3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Immunoprecipitation - Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)

UBE2C was immunoprecipitated from 0.35 mg SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg with ab252940 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252940 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: SW480 whole cell lysate 10µg.

Lane 2: ab252940 IP in SW480 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252940 in SW480 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252940).
Anti-UBE2C antibody [EPR23165-31] - BSA and Azide free (ab269592)