Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBE2C antibody [EPR23165-31] (ab252940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23165-31] to UBE2C
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UBE2C antibody [EPR23165-31]
    See all UBE2C primary antibodies

  • Description

    Rabbit monoclonal [EPR23165-31] to UBE2C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T-47D, SW480, NIH:OVCAR-3, HeLa, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and rat spleen tissue lysate. IHC-P: Human gastric cancer tissue and colon tissue. Mouse and rat colon tissue. ICC/IF: SW480 and RAW 264.7 cells. IP: SW480 and NIH:OVCAR-3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasm and nuclear staining in proliferative cells of human gastric cancer is observed (PMID:15208666, 27349176). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling UBE2C with ab252940 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in partial RAW 264.7 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Western blot - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Western blot - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    All lanes : Anti-UBE2C antibody [EPR23165-31] (ab252940) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate at 20 µg
    Lane 2 : Rat spleen lysate at 20 µg
    Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27349176, 17217624).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in proliferative cells of mouse colon is observed (PMID: 15208666). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunoprecipitation - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunoprecipitation - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    UBE2C was immunoprecipitated from 0.35 mg NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate 10µg with ab252940 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252940 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH:OVCAR-3 whole cell lysate 10µg.

    Lane 2: ab252940 IP in NIH:OVCAR-3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252940 in NIH:OVCAR-3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling UBE2C with ab252940 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in partial SW480 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in proliferative cells of rat colon is observed (PMID: 15208666). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Western blot - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Western blot - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    All lanes : Anti-UBE2C antibody [EPR23165-31] (ab252940) at 1/1000 dilution

    Lane 1 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
    Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27349176, 17217624).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling UBE2C with ab252940 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasm and nuclear staining in proliferative cells of human colon is observed (PMID: 15208666). The section was incubated with ab252940 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunoprecipitation - Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Immunoprecipitation - Anti-UBE2C antibody [EPR23165-31] (ab252940)

    UBE2C was immunoprecipitated from 0.35 mg SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg with ab252940 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252940 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: SW480 whole cell lysate 10µg.

    Lane 2: ab252940 IP in SW480 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252940 in SW480 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Anti-UBE2C antibody [EPR23165-31] (ab252940)
    Anti-UBE2C antibody [EPR23165-31] (ab252940)

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