Anti-Keap1 antibody [1B4] (ab119403)
![Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132639-anti-keap1-antibody-1b4-western-blot.jpg "Anti-Keap1 antibody [1B4] (ab119403)")
Key features and details
- Mouse monoclonal [1B4] to Keap1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Keap1 antibody [1B4]
See all Keap1 primary antibodies -
Description
Mouse monoclonal [1B4] to Keap1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length Human Keap1 produced in HEK293T cells (NP_987096).
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Keap1 overexpress plasmid; Jurkat and HeLa cells; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues.
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General notes
Dilute in PBS (pH7.3) before use.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab119403 was purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
1B4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Keap1 antibody [1B4] (ab119403)All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189) -
![Western blot - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132638-anti-keap1-antibody-1b4-western-blot.jpg)
Western blot - Anti-Keap1 antibody [1B4] (ab119403)All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132637-anti-keap1-antibody-1b4-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [1B4] (ab119403)ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132636-anti-keap1-antibody-1b4-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [1B4] (ab119403)ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132635-anti-keap1-antibody-1b4-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [1B4] (ab119403)ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry. -
![Flow Cytometry - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132634-anti-keap1-antibody-1b4-flow-cytometry.jpg)
Flow Cytometry - Anti-Keap1 antibody [1B4] (ab119403)ab119403 at 1/100 dilution staining Keap1 in HEK293T cells transfected with either pCMV6-ENTRY Keap1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry. -
![Flow Cytometry - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132633-anti-keap1-antibody-1b4-flow-cytometry.jpg)
Flow Cytometry - Anti-Keap1 antibody [1B4] (ab119403)ab119403 at 1/100 dilution staining Keap1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue). -
![Flow Cytometry - Anti-Keap1 antibody [1B4] (ab119403)](https://www.abcam.com/ps/products/119/ab119403/Images/ab119403-132632-anti-keap1-antibody-1b4-flow-cytometry.jpg)
Flow Cytometry - Anti-Keap1 antibody [1B4] (ab119403)ab119403 at 1/100 dilution staining Keap1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
