Anti-Keap1 antibody (ab139729)
Key features and details
- Rabbit polyclonal to Keap1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Keap1 antibody
See all Keap1 primary antibodies -
Description
Rabbit polyclonal to Keap1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Keap1 aa 574-624 (C terminal). The exact sequence is proprietary. Synthetic peptide corresponding to 19 amino acids near the C-terminus of Human Keap1.
Database link: NP_987096 -
Positive control
- WB: Recombinant Human Keap1 protein (ab132384), Daudi, HeLa, and K562 cell lysate, and human lung tissue lysate. IHC-P: Human lung tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Keap1 antibody (ab139729) at 2 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KEAP1 knockout HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 70 kDaIncubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.
Incubated with anti-beta actin at 1µg/ml.
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Immunofluorescence of Keap1 in human lung tissue with ab139727 at 20µg/mL.
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Lane 1 : Anti-Keap1 antibody (ab139729) at 1 µg/ml
Lane 2 : Anti-Keap1 antibody (ab139729) at 2 µg/ml
All lanes : Human lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 70 kDa