Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22664-26] to Keap1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free
    See all Keap1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22664-26] to Keap1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lung tissue lysate; Wild-type HAP1, NK-92 and HepG2 whole cell lysates; mouse E14.5 liver tissue lysate. IHC-P: Human lung carcinoma tissue; mouse kidney tissue.

  • General notes

    Ab256813 is the carrier-free version of ab227828. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256813 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)
    Western blot - Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)
    All lanes : Anti-Keap1 antibody [EPR22664-26] (ab227828) at 1/1000 dilution

    Lane 1 : Human lung tissue lysate
    Lane 2 : Wild-type HAP1 whole cell lysate
    Lane 3 : Keap1 knockout HAP1 whole cell lysate
    Lane 4 : NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate
    Lane 5 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 6 : Mouse E14.5 liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 2-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 3 minutes


    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    ab227828 was shown to specifically react with Keap1 in wild-type HAP1 cells as signal was lost in Keap1 knockout cells. Wild-type and Keap1 knockout samples were subjected to SDS-PAGE. ab227828 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23242280, PMID: 20404090).

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227828).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Keap1 with ab227828 at 1/500 dilution (0.97 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on kidney tubules of mouse kidney (PMID: 28324005) is observed. The section was incubated with ab227828 for 30 mins at room temperature.  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227828).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Keap1 with ab227828 at 1/500 dilution (0.97 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tumor cells of human lung carcinoma (PMID: 20534738) is observed. The section was incubated with ab227828 for 30 mins at room temperature.  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227828).

  • Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)
    Anti-Keap1 antibody [EPR22664-26] - BSA and Azide free (ab256813)

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