Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)
![Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)](https://www.abcam.com/ps/products/255/ab255865/Images/ab255865-349596-ataxin3antibodyepr22418147westernblotmouserat.jpg "Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22418-147] to Ataxin 3 - BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free
See all Ataxin 3 primary antibodies -
Description
Rabbit monoclonal [EPR22418-147] to Ataxin 3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-87 MG, NIH/3T3 and C6 whole cell lysate; Human brain, spleen and cerebellum tissue lysate; Mouse skin and spleen tissue lysate; Rat Brain and cerebellum tissue lysate.
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General notes
Ab255865 is the carrier-free version of ab221143. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255865 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22418-147 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)All lanes : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 4 : Mouse skin tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-2: 26 seconds
Lanes 3-4: 3 minutes
Lane 5: 15 seconds
Lane 6: 3 minutes
Lane 7: 59 seconds
The molecular weight is consistent with what has been described in the literature (PMID: 30455355).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221143).
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![ELISA - Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)](https://www.abcam.com/ps/products/255/ab255865/Images/ab255865-2-anti-ataxin-3-antibody-epr22418-147-elisa-human-atxn31.jpg)
ELISA - Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)indirect ELISA using ab221143 at varying antibody concentrations (1000-0 ng/ml) and Human ATXN3 antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221143).
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![Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)](https://www.abcam.com/ps/products/255/ab255865/Images/ab255865-3-benefits-of-recombinant-antibodies.png)
Anti-Ataxin 3 antibody [EPR22418-147] - BSA and Azide free (ab255865)
