Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free
See all ATP6V0D1/P39 primary antibodies
Description

Rabbit monoclonal [EPR18320-38] to ATP6V0D1/P39 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251387 is the carrier-free version of ab202899 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251387 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as ATP6V0D1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR18320-38
Isotype

IgG
Research areas

Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

All lanes : Anti-ATP6V0D1/P39 antibody [EPR18320-38] (ab202899) at 1/10000 dilution

Lane 1 : Human fetal kidney
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma)
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line)
Lane 4 : A431 (Human epidermoid carcinoma)

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 1 minute

This data was developed using ab202899, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

Anti-ATP6V0D1/P39 antibody [EPR18320-38] (ab202899) at 1/2000 dilution + Human fetal brain at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 5 seconds

This data was developed using ab202899, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

All lanes : Anti-ATP6V0D1/P39 antibody [EPR18320-38] (ab202899) at 1/2000 dilution

Lane 1 : Mouse kidney
Lane 2 : Mouse spleen
Lane 3 : Rat spleen
Lane 4 : C6 (Rat glial tumor cells)
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma)
Lane 6 : NIH/3T3 (mouse embryo fibroblast cells)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 5 seconds

This data was developed using ab202899, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ATP6V0D1/P39 with ab202899 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling ATP6V0D1/P39 with ab202899 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V0D1/P39 with ab202899 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Mouse kidney tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V0D1/P39 with ab202899 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATP6V0D1/P39 with ab202899 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor^® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab202899 at 1/500 dilution followed by ab150120 (Alexa Fluor^® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor^® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast adenocarcinoma) cells labeling ATP6V0D1/P39 with ab202899 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on MCF-7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor^® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab202899 at 1/500 dilution followed byab150120 (Alexa Fluor^® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor^® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Flow Cytometry - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HeLa cells labelling ATP6V0D1/P39 with ab202899 at 1/800 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)

This data was developed using ab202899, the same antibody clone in a different buffer formulation.

ATP6V0D1/P39 was immunoprecipitated from 1mg of MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate with ab202899 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202899 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Input MCF-7 whole cell lysate (10 µg). Lane 2: MCF-7 whole cell lysate following precipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202899 in MCF-7 whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-ATP6V0D1/P39 antibody [EPR18320-38] - BSA and Azide free (ab251387)