Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22418-147] to Ataxin 3
Suitable for: WB, ELISA
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-Ataxin 3 antibody [EPR22418-147]
See all Ataxin 3 primary antibodies
Description

Rabbit monoclonal [EPR22418-147] to Ataxin 3
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U-87 MG, NIH/3T3 and C6 whole cell lysate; Human brain, spleen and cerebellum tissue lysate; Mouse skin and spleen tissue lysate; Rat brain and cerebellum tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22418-147
Isotype

IgG
Research areas

Western blot - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

All lanes : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution

Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 4 : Mouse skin tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : Rat cerebellum tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure time:

Lanes 1-2: 26 seconds

Lanes 3-4: 3 minutes

Lane 5: 15 seconds

Lane 6: 3 minutes

Lane 7: 59 seconds

The molecular weight is consistent with what has been described in the literature (PMID: 30455355).
ELISA - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

Indirect ELISA using ab221143 at varying antibody concentrations (1000-0 ng/ml) and Human ATXN3 antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.
Western blot - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

Lanes 1-3 : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/1000 dilution
Lane 4 : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution

Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Human cerebellum tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 42 kDa
Observed band size: 45, 35 kDa why is the actual band size different from the predicted?

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure times:

Lane 1: 10 seconds

Lanes 2-3: 125 seconds

Lane 4: 5 seconds

The full-length ataxin-3 (45kDa) and a 35-kDa band, likely to be an ataxin-3 isoform or fragment, are observed. The molecular weights are consistent with what have been described in the literature (PMID: 30455355 and UniProt annotation).
Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

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