Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22418-147] to Ataxin 3
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Ataxin 3 antibody [EPR22418-147]
    See all Ataxin 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22418-147] to Ataxin 3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG, NIH/3T3 and C6 whole cell lysate; Human brain, spleen and cerebellum tissue lysate; Mouse skin and spleen tissue lysate; Rat brain and cerebellum tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    Western blot - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    All lanes : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line), whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
    Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 4 : Mouse skin tissue lysate
    Lane 5 : Mouse spleen tissue lysate
    Lane 6 : Rat brain tissue lysate
    Lane 7 : Rat cerebellum tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:

    Lanes 1-2: 26 seconds

    Lanes 3-4: 3 minutes

    Lane 5: 15 seconds

    Lane 6: 3 minutes

    Lane 7: 59 seconds

    The molecular weight is consistent with what has been described in the literature (PMID: 30455355).

  • ELISA - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    ELISA - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    Indirect ELISA using ab221143 at varying antibody concentrations (1000-0 ng/ml) and Human ATXN3 antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.
  • Western blot - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    Western blot - Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    Lanes 1-3 : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/1000 dilution
    Lane 4 : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Human spleen tissue lysate
    Lane 4 : Human cerebellum tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 45, 35 kDa why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure times:

    Lane 1: 10 seconds

    Lanes 2-3: 125 seconds

    Lane 4: 5 seconds

    The full-length ataxin-3 (45kDa) and a 35-kDa band, likely to be an ataxin-3 isoform or fragment, are observed. The molecular weights are consistent with what have been described in the literature (PMID: 30455355 and UniProt annotation).

  • Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
    Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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