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Signal Transduction Metabolism Energy Metabolism

Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13134-23] to NCF1/p47-phox
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-NCF1/p47-phox antibody [EPR13134-23]
    See all NCF1/p47-phox primary antibodies
  • Description

    Rabbit monoclonal [EPR13134-23] to NCF1/p47-phox
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Raji and Ramos cell lysates; Human spleen tissue.
  • General notes

    Previously labelled as NCF1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13134-23
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
    Western blot - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
    All lanes : Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090) at 1/2000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
    Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)

    Western blot analysis of Raji cell lysate immunoprecipitated with ab181090 at 1/20 dilution (Lane 1). Lane 2: Negative control.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling NCF1/p47-phox with ab181090 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
    Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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