Anti-NCF1/p47-phox antibody (ab166930)
Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NCF1/p47-phox antibody
See all NCF1/p47-phox primary antibodies -
Description
Goat polyclonal to NCF1/p47-phox -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Dog, Pig, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Mouse NCF1/p47-phox aa 217-230 (internal sequence) (Cysteine residue).
Sequence:DEAEDPDPNYAGEP
Database link: NP_035006.3 -
Positive control
- WB: Daudi cell lydates ICC/IF: RAW264.7 and NIH3T3 cells
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General notes
Previously labelled as NCF1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab166930 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCF1/p47-phox antibody (ab166930)Anti-NCF1/p47-phox antibody (ab166930) at 1 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 45 kDaDetected by chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (ab166930)Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL). -
Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (ab166930)Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
