Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
Key features and details
- Rabbit polyclonal to NCF1/p47-phox (phospho S304)
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-NCF1/p47-phox (phospho S304) antibody
See all NCF1/p47-phox primary antibodies -
Description
Rabbit polyclonal to NCF1/p47-phox (phospho S304) -
Host species
Rabbit -
Specificity
Detects NCF1/p47-phox only when phosphorylated at serine 304.
-
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey
-
Immunogen
Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S304).
Sequence:R-S-Sp-I-R
-
Positive control
- COS7 cells
-
General notes
Previously labelled as NCF1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)All lanes : Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution
Lane 1 : extracts from COS7 cells treated
with UV (15mins)
Lane 2 : A549 cells extracts
Lane 3 : A549 cells extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
