Anti-Cbx8 antibody [EPR23800-14] (ab259849)
![Anti-Cbx8 antibody [EPR23800-14] (ab259849)](https://www.abcam.com/ps/products/259/ab259849/Images/ab259849-451597-anti-cbx8-antibody-epr2380014-western-blot-adenocarcinoma.jpg "Anti-Cbx8 antibody [EPR23800-14] (ab259849)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23800-14] to Cbx8
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cbx8 antibody [EPR23800-14]
See all Cbx8 primary antibodies -
Description
Rabbit monoclonal [EPR23800-14] to Cbx8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa; 293T; Huh7; NIH/3T3 and C6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23800-14 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cbx8 antibody (ab259849)All lanes : Anti-Cbx8 antibody [EPR23800-14] (ab259849) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 43 kDa
Observed band size: 48/43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Incubation for primary antibody was carried out overnight at 4 degree.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 48 seconds
