Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cbx8 antibody [EPR23800-14] (ab259849)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23800-14] to Cbx8
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cbx8 antibody [EPR23800-14]
    See all Cbx8 primary antibodies

  • Description

    Rabbit monoclonal [EPR23800-14] to Cbx8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa; 293T; Huh7; NIH/3T3 and C6 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23800-14

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cbx8 antibody (ab259849)
    Western blot - Anti-Cbx8 antibody (ab259849)
    All lanes : Anti-Cbx8 antibody [EPR23800-14] (ab259849) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 43 kDa
    Observed band size: 48/43 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Incubation for primary antibody was carried out overnight at 4 degree. 

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 48 seconds

     

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