Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)
![Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)](https://www.abcam.com/ps/products/109/ab109453/Images/ab109453-265987-anti-p95-nbs1-phospho-s343-antibody-ep178-western-blot.jpg "Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP178] to p95/NBS1 (phospho S343)
- Suitable for: Dot blot, WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-p95/NBS1 (phospho S343) antibody [EP178]
See all p95/NBS1 primary antibodies -
Description
Rabbit monoclonal [EP178] to p95/NBS1 (phospho S343) -
Host species
Rabbit -
Specificity
ab109453 only detects p95/NBS1 phosphorylated on Serine 343.
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Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HeLa cell lysates (treated and untreated with Etopside). ICC: Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as p95 NBS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP178 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)All lanes : Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells whole cell lysates
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were treated with Etopside whole cell lysates
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were treated with Etopside whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry - Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)](https://www.abcam.com/ps/products/109/ab109453/Images/ab109453-4-anti-p95-nbs1-phospho-s343-antibody-ep178-immunocytochemistry-jurkat-human.jpg)
Immunocytochemistry - Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) labeling p95/NBS1 with purified ab109453 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.27 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Dot Blot - Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)](https://www.abcam.com/ps/products/109/ab109453/Images/ab109453-255421-anti-p95-nbs1-phospho-s343-antibody-ep178-dot-blot.jpg)
Dot Blot - Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)Dot blot analysis of p95/NBS1 (pS343) peptide (Lane 1) and p95/NBS1 non-phospho peptide (Lane 2) labelling p95/NBS1 (phospho S343) with ab109453 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)](https://www.abcam.com/ps/products/109/ab109453/Images/ab109453-5-benefits-of-recombinant-antibodies.png)
Anti-p95/NBS1 (phospho S343) antibody [EP178] (ab109453)
