Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2470Y] to p95/NBS1 (phospho S432)
- Suitable for: IHC-P, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y]
See all p95/NBS1 primary antibodies -
Description
Rabbit monoclonal [EPR2470Y] to p95/NBS1 (phospho S432) -
Host species
Rabbit -
Specificity
ab75778 detects p95/NBS1 that is phosphorylated on serine 432.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
corresponding to Human p95/NBS1.
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Positive control
- HeLa cell lysates, untreated or treated with doxorubincin and human testis tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as p95 NBS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2470Y -
Isotype
IgG -
Research areas
Images
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Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: p95/NBS1 (pS432) phospho peptide
Lane 2 sample: p95/NBS1 non-phospho peptide
Exposure time: 3 minutes
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ab75778 staining p95/NBS1 (phospho S432) in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
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ab75778 at 1/100-1/250 dilution staining p95/NBS1 in human testis by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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