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Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2470Y] to p95/NBS1 (phospho S432)
  • Suitable for: IHC-P, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y]
    See all p95/NBS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2470Y] to p95/NBS1 (phospho S432)
  • Host species

    Rabbit
  • Specificity

    ab75778 detects p95/NBS1 that is phosphorylated on serine 432.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human p95/NBS1.

  • Positive control

    • HeLa cell lysates, untreated or treated with doxorubincin and human testis tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as p95 NBS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2470Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Images

  • Dot Blot - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)
    Dot Blot - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: p95/NBS1 (pS432) phospho peptide

    Lane 2 sample: p95/NBS1 non-phospho peptide

    Exposure time: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

    ab75778 staining p95/NBS1 (phospho S432) in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

    ab75778 at 1/100-1/250 dilution staining p95/NBS1 in human testis by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)
    Anti-p95/NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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