Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)
![Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967-62767-anti-heavy-chain-cardiac-myosin-antibody-ba-g5-western-blot.jpg "Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)")
Key features and details
- Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Overview
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Product name
Anti-heavy chain cardiac Myosin antibody [BA-G5]
See all heavy chain cardiac Myosin primary antibodies -
Description
Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Tissue, cells or virus corresponding to Cow heavy chain cardiac Myosin.
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
BA-G5 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
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![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967-62765-anti-heavy-chain-cardiac-myosin-antibody-ba-g5-western-blot.jpg)
Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 48 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
