Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYH6 antibody [3-48 G5C7] (ab207926)

Key features and details

  • Mouse monoclonal [3-48 G5C7] to MYH6
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-MYH6 antibody [3-48 G5C7]

  • Description

    Mouse monoclonal [3-48 G5C7] to MYH6

  • Host species

    Mouse

  • Specificity

    This antibody recognizes alpha and beta type MYH6 fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains Specific for: human, rat and mouse; other species not tested but it is expected to also recognize and heavy chain from canine, bovine and rabbit cardiac tissues.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Human heavy chain cardiac Myosin. Human ventricular myosin.

  • Positive control

    • WB: Human fetal, human adult, mouse and rat heart tissue lysates, human, mouse and rat skeletal muscle tissue lysates. IHC-P: Human, mouse and rat heart tissues.

  • General notes

    Molecular weight if IgG: according to SDS-PAGE under mercaptoethanol (reduced conditions) 153.8 kd. Heavy chain: 49.7 kd light chain: 27.2 kd. Molecular weight of F(ab')2: according to SDS-PAGE under mercaptoethanol (reduced conditions) 116 kd. Heavy chain: 30.8 kd light chain: 27.2 kd

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (ab207926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (ab207926)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse heart tissue labeling MYH6 with ab207926 at 1 µg/ml.

  • Western blot - Anti-MYH6 antibody [3-48 G5C7] (ab207926)
    Western blot - Anti-MYH6 antibody [3-48 G5C7] (ab207926)
    All lanes : Anti-MYH6 antibody [3-48 G5C7] (ab207926) at 1/2000 dilution

    Lane 1 : Human fetal heart tissue lysate at 20 µg
    Lane 2 : Human fetal heart tissue lysate at 5 µg
    Lane 3 : Human adult heart tissue lysate at 20 µg
    Lane 4 : Mouse heart tissue lysate at 20 µg
    Lane 5 : Rat heart tissue lysate at 20 µg
    Lane 6 : Human skeletal muscle tissue lysate at 20 µg
    Lane 7 : Mouse skeletal muscle tissue lysate at 20 µg
    Lane 8 : Rat skeletal muscle tissue lysate at 20 µg
    Lane 9 : Human liver tissue lysate at 20 µg

    Predicted band size: 223 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (ab207926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (ab207926)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human heart tissue labeling MYH6 with ab207926 at 1 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (ab207926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (ab207926)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat heart tissue labeling MYH6 with ab207926 at 1 µg/ml.

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