Anti-Myosin Phosphatase antibody (ab70809)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin Phosphatase antibody
See all Myosin Phosphatase primary antibodies -
Description
Rabbit polyclonal to Myosin Phosphatase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 842) of human Myosin Phosphatase (NP_002471.1)
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Positive control
- Whole cell lysate from HeLa or 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Myosin Phosphatase antibody (ab70809)All lanes : Anti-Myosin Phosphatase antibody (ab70809) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Myosin Phosphatase antibody (ab70809)1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
