Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase (phospho T853)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin Phosphatase (phospho T853) antibody
See all Myosin Phosphatase primary antibodies -
Description
Rabbit polyclonal to Myosin Phosphatase (phospho T853) -
Host species
Rabbit -
Specificity
Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat) -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 853 (RSTPGV)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)All lanes : Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunizing phosphopeptide
Predicted band size: 115 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
