Anti-SMC1A antibody [BL-205-2G8] (ab243875)
![Anti-SMC1A antibody [BL-205-2G8] (ab243875)](https://www.abcam.com/ps/products/243/ab243875/Images/ab243875-4-ab243875ICC.jpg "Anti-SMC1A antibody [BL-205-2G8] (ab243875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-205-2G8] to SMC1A
- Suitable for: ICC, IHC-P, IP, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SMC1A antibody [BL-205-2G8]
See all SMC1A primary antibodies -
Description
Rabbit monoclonal [BL-205-2G8] to SMC1A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human SMC1A aa 1200-1233 (C terminal). The exact sequence is proprietary. NP_006297.2 and Gene ID 8243.
Database link: Q14683 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-205-2G8 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-SMC1A antibody [BL-205-2G8] (ab243875)
Formaldehyde-fixed HCT-116 (human colorectal carcinoma cell line) cells labeling SMC1A using ab243875 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (ab243875)](https://www.abcam.com/ps/products/243/ab243875/Images/ab243875-3-ab243875IHCP.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody [BL-205-2G8] (ab243875)Formalin-fixed, paraffin-embedded human Breast carcinoma tissue stained for SMC1A using ab243875 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
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![Immunoprecipitation - Anti-SMC1A antibody [BL-205-2G8] (ab243875)](https://www.abcam.com/ps/products/243/ab243875/Images/ab243875-2-ab243875IP.jpg)
Immunoprecipitation - Anti-SMC1A antibody [BL-205-2G8] (ab243875)SMC1A was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243875 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243875 at 1/1000 dilution.
Lane 1: ab243875 IP in HEK-293T whole cell lysate.
Lane 2: Contol IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-SMC1A antibody [BL-205-2G8] (ab243875)](https://www.abcam.com/ps/products/243/ab243875/Images/ab243875-1-ab243875WB.jpg)
Western blot - Anti-SMC1A antibody [BL-205-2G8] (ab243875)Western blot analysis using ab243875 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: MCF7 whole cell lysate (15 µg).
Lane 4: HepG2 whole cell lysate (15 µg).
Lane 5: A549 whole cell lysate (15 µg).
Lane 6: SW620 whole cell lysate (15 µg).
Lane 7: SK whole cell lysate (15µg).
Lane 8: Jurkat whole cell lysate (15 µg).
Lane 9: TCMK-1 whole cell lysate (15 µg).
Lane 10: NIH/3T3 whole cell lysate (15 µg).
Lane 11: CT26 whole cell lysate (15 µg)
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
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![Anti-SMC1A antibody [BL-205-2G8] (ab243875)](https://www.abcam.com/ps/products/243/ab243875/Images/ab243875-5-benefits-of-recombinant-antibodies.png)
Anti-SMC1A antibody [BL-205-2G8] (ab243875)
