Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20904-32] to SLFN12 - BSA and Azide free
  • Suitable for: WB, IHC-Fr, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free
    See all SLFN12 primary antibodies

  • Description

    Rabbit monoclonal [EPR20904-32] to SLFN12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HeLa whole cell lysate treated with DMSO or 17ß-estradiol. IHC-Fr: Human placenta tissue. Flow Cyt: HeLa cells.

  • General notes

    Ab256300 is the carrier-free version of ab234418. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256300 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20904-32

  • Isotype

    IgG

Images

  • Immunohistochemistry (Frozen sections) - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)
    Immunohistochemistry (Frozen sections) - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen human placenta tissue labeling SLFN12 with ab234418 at 1/100 dilution (green), followed by Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 secondary at a 1/100 dilution. Positive staining in syncytiotrophoblast of human placenta is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 used at a 1/100 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).


    The image is kindly provided by Dr Xiaodong Wang.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234418).
  • Flow Cytometry - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)
    Flow Cytometry - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLFN12 with ab234418 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234418).
  • Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)
    Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (ab256300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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