Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLFN12 antibody [EPR20904-32] (ab234418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20904-32] to SLFN12
  • Suitable for: WB, IHC-Fr, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SLFN12 antibody [EPR20904-32]
    See all SLFN12 primary antibodies

  • Description

    Rabbit monoclonal [EPR20904-32] to SLFN12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Recombinant fragment
    IHC-Fr
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HeLa whole cell lysate treated with DMSO or 17ß-estradiol. HEK-293 transfected with SLFN12 (Q8IYM2) (WT) expression vector containing HA-tag®, whole cell lysate. IHC-Fr: Human placenta tissue. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20904-32

  • Isotype

    IgG

Images

  • Immunohistochemistry (Frozen sections) - Anti-SLFN12 antibody [EPR20904-32] (ab234418)
    Immunohistochemistry (Frozen sections) - Anti-SLFN12 antibody [EPR20904-32] (ab234418)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen human placenta tissue labeling SLFN12 with ab234418 at 1/100 dilution (green), followed by Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 secondary at a 1/100 dilution. Positive staining in syncytiotrophoblast of human placenta is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 used at a 1/100 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

    The image is kindly provided by Dr Xiaodong Wang.

  • Western blot - Anti-SLFN12 antibody [EPR20904-32] (ab234418)
    Western blot - Anti-SLFN12 antibody [EPR20904-32] (ab234418)
    All lanes : Anti-SLFN12 antibody [EPR20904-32] (ab234418) at 1/500 dilution

    Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO for 12 hours, whole cell lysate
    Lane 2 : Wild-type HeLa treated with 17ß-estradiol (5 µM) for 12 hours, whole cell lysate
    Lane 3 : SLFN12 knockout HeLa treated with DMSO for 12 hours, whole cell lysate
    Lane 4 : SLFN12 knockout HeLa treated with 17ß-estradiol (5 µM) for 12 hours, whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% Defatted milk.

    The image is kindly provided by Dr Xiaodong Wang.

    ab234418 was shown to specifically react with SLFN12 in wild-type Hela cells as signal was lost in SLFN12 knockout cells. Wild-type and SLFN12 knockout samples were subjected to SDS-PAGE. ab234418 and anti-GAPDH antibody were incubated 8 hours at 4 °C at 1/500 dilution and 1/2000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (whole molecule), Peroxidase conjugated secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

  • Western blot - Anti-SLFN12 antibody [EPR20904-32] (ab234418)
    Western blot - Anti-SLFN12 antibody [EPR20904-32] (ab234418) SLFN12 plasmid was kindly provided by an anonymous collaborator.
    All lanes : Anti-SLFN12 antibody [EPR20904-32] (ab234418) at 1/500 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containing HA- tag® whole cell lysates
    Lane 2 : HEK-293 (human embryonic kidney) transfected with SLFN12 (Q8IYM2) (WT) expression vector containing HA-tag®, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Developed using the ECL technique.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry - Anti-SLFN12 antibody [EPR20904-32] (ab234418)
    Flow Cytometry - Anti-SLFN12 antibody [EPR20904-32] (ab234418)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLFN12 with ab234418 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Anti-SLFN12 antibody [EPR20904-32] (ab234418)
    Anti-SLFN12 antibody [EPR20904-32] (ab234418)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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