Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)
![Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)](https://www.abcam.com/ps/products/75/ab75768/Images/ab75768-90830-anti-smc1-phospho-s957-antibody-epr2857y-western-blot.jpg "Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2857Y] to SMC1A (phospho S957)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SMC1A (phospho S957) antibody [EPR2857Y]
See all SMC1A primary antibodies -
Description
Rabbit monoclonal [EPR2857Y] to SMC1A (phospho S957) -
Host species
Rabbit -
Specificity
Detects SMC1A only when phosphorylated on serine 957.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human SMC1A.
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Positive control
- Hela cell lysate; human breast carcinoma or tonsil tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2857Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)All lanes : Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution
Lane 1 : Hela cells lysate, untreated
Lane 2 : Hela cells lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)](https://www.abcam.com/ps/products/75/ab75768/Images/ab75768-90829-anti-smc1-phospho-s957-antibody-epr2857y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)ab75768 at 1/100 dilution staining SMC1A in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)](https://www.abcam.com/ps/products/75/ab75768/Images/ab75768-90828-anti-smc1-phospho-s957-antibody-epr2857y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)ab75768 at 1/100 dilution staining SMC1A in human tonsil tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)](https://www.abcam.com/ps/products/75/ab75768/Images/ab75768-4-benefits-of-recombinant-antibodies.png)
Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768)
