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Anti-SMC1A antibody (ab140493)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-SMC1A antibody (ab140493)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to SMC1A
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SMC1A antibody
    See all SMC1A primary antibodies
  • Description

    Goat polyclonal to SMC1A
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human SMC1A aa 1183-1233.
    Sequence:

    QAIVISLK EEFYTKAESL IGVYPEQGDC VISKVLTFDL TKYPDANPNP NEQ

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat whole cell lysate (ab7899); NIH3T3 cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab140493 was affinity purified using an epitope specific to SMC1A immobilized on a solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Images

  • Western blot - Anti-SMC1A antibody (ab140493)
    Western blot - Anti-SMC1A antibody (ab140493)
    All lanes : Anti-SMC1A antibody (ab140493) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa


    Exposure time: 3 minutes
  • Western blot - Anti-SMC1A antibody (ab140493)
    Western blot - Anti-SMC1A antibody (ab140493)
    All lanes : Anti-SMC1A antibody (ab140493) at 0.4 µg/ml

    Lane 1 : NIH3T3 whole cell lysate at 50 µg
    Lane 2 : NIH3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-SMC1A antibody (ab140493)
    Immunoprecipitation - Anti-SMC1A antibody (ab140493)
    Immunoprecipitation of SMC1A from Hela whole cell lysate by ab140493 at 6 &microg/mg lysate (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control. Detection: chemiluminescence. Exposure time: 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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