Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)
![Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)](https://www.abcam.com/ps/products/81/ab81306/Images/ab81306-96519-anti-smc1-phospho-s966-antibody-ep2858y-western-blot.jpg "Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)")
Key features and details
- Rabbit monoclonal [EP2858Y] to SMC1A (phospho S966)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMC1A (phospho S966) antibody [EP2858Y]
See all SMC1A primary antibodies -
Description
Rabbit monoclonal [EP2858Y] to SMC1A (phospho S966) -
Host species
Rabbit -
Specificity
This antibody detects SMC1A phosphorylated on Serine 966.
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Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
corresponding to Human SMC1A.
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Positive control
- HeLa whole cell lysate (ab150035). HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2858Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)All lanes : Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306) at 1/1000 dilution
Lane 1 : HeLa cell lysates un-treated
Lane 2 : HeLa cell lysates treated with hydroxyurea
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)](https://www.abcam.com/ps/products/81/ab81306/Images/ab81306-96518-anti-smc1-phospho-s966-antibody-ep2858y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMC1A (phospho S966) antibody [EP2858Y] (ab81306)Immunofluorescent staining of HeLa cells using ab81306 at a 1/250 dilution.
