Anti-FHL3 antibody [EPR14243(B)] (ab180509)
![Anti-FHL3 antibody [EPR14243(B)] (ab180509)](https://www.abcam.com/ps/products/180/ab180509/Images/ab180509-215676-anti-fhl3-antibody-epr14243b-western-blot.jpg "Anti-FHL3 antibody [EPR14243(B)] (ab180509)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14243(B)] to FHL3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Overview
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Product name
Anti-FHL3 antibody [EPR14243(B)]
See all FHL3 primary antibodies -
Description
Rabbit monoclonal [EPR14243(B)] to FHL3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle, K562 cell line & Rat muscle lysates, Human muscle tissue, A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14243(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)All lanes : Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Rat muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 31 kDaBlocking buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (ab180509)](https://www.abcam.com/ps/products/180/ab180509/Images/ab180509-215678-anti-fhl3-antibody-epr14243b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (ab180509)Immunofluorescence analysis of A673 cells labeling FHL3 with ab180509 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/250 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (ab180509)](https://www.abcam.com/ps/products/180/ab180509/Images/ab180509-215677-anti-fhl3-antibody-epr14243b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (ab180509)Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling FHL3 with ab180509 at 1/500 dilution. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)](https://www.abcam.com/ps/products/180/ab180509/Images/ab180509-215675-anti-fhl3-antibody-epr14243b-western-blot.jpg)
Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/5000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG HRP (ab136636) at 1/500 dilution
Predicted band size: 31 kDaBlocking buffer: 5% NFDM/TBST
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![Anti-FHL3 antibody [EPR14243(B)] (ab180509)](https://www.abcam.com/ps/products/180/ab180509/Images/ab180509-5-benefits-of-recombinant-antibodies.png)
Anti-FHL3 antibody [EPR14243(B)] (ab180509)
