Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FHL3 antibody [EPR14243(B)] (ab180509)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14243(B)] to FHL3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-FHL3 antibody [EPR14243(B)]
    See all FHL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14243(B)] to FHL3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle, K562 cell line & Rat muscle lysates, Human muscle tissue, A673 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    All lanes : Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Rat muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 31 kDa



    Blocking buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (ab180509)

    Immunofluorescence analysis of A673 cells labeling FHL3 with ab180509 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/250 dilution. Fixative: 4% paraformaldehyde.  Counter stain: Dapi

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (ab180509)

    Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling FHL3 with ab180509 at 1/500 dilution. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/5000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG HRP (ab136636) at 1/500 dilution

    Predicted band size: 31 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-FHL3 antibody [EPR14243(B)] (ab180509)
    Anti-FHL3 antibody [EPR14243(B)] (ab180509)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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