Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (ab279642)
![Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (ab279642)](https://www.abcam.com/ps/products/279/ab279642/Images/ab279642-1-anti-histone-h11-antibody-epr2319114-immunohistochemistry-human-testis.jpg "Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (ab279642)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23191-14] to Histone H1.1 - BSA and Azide free
- Suitable for: WB, Dot blot, ICC, Flow Cyt (Intra), IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free
See all Histone H1.1 primary antibodies -
Description
Rabbit monoclonal [EPR23191-14] to Histone H1.1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, ICC, Flow Cyt (Intra), IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-468 whole cell lysate. IHC-P: Human testis and hepatocelluar carcinoma tissue. ICC: MDA-MB-468 cells. Flow Cyt: MDA-MB-468 cells.
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General notes
ab279642 is the carrier-free version of ab254394. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab279642 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23191-14 -
Isotype
IgG
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)
This data was developed using ab254394, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Histone H1.1 with ab254394 at 1/2000 (0.342 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human testis. The section was incubated with ab254394 overnight at 4°C. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Immunocytochemistry - Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)This data was developed using ab254394, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-468 cells labelling Histone H1.1 with ab254394 at 1/1000 (0.683 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MDA-MB-468 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow Cytometry - Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)This data was developed using ab254394, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-468 (Human breast adenocarcinoma epithelial cell) cells labelling Histone H1.1 with ab254394 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Western blot - Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)All lanes : Anti-Histone H1.1 antibody [EPR23191-14] (ab254394) at 1/1000 dilution
Lane 1 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 22 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?This data was developed using 254394, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: MDA-MB-231.
Exposure time: 3 minutes.
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Dot Blot - Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)This data was developed using ab254394, the same antibody clone in a different buffer formulation.
Dot blot analysis of Histone H1.1 using ab254394 at 1/1000 (0.683 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate (ab97051) at 1/100,000 dilution.
Lane 1: Histone H1.1 peptide
Lane 2: Histone H1.2 peptide
Lane 3: Histone H1.3 peptide
Lane 4: Histone H1.4 peptide
Exposure time: 3 minutes.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)This data was developed using ab254394, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocelluar carcinoma tissue labelling Histone H1.1 with ab254394 at 1/2000 (0.342 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on human hepatocelluar carcinoma. The section was incubated with ab254394 overnight at 4°C. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Anti-Histone H1.1 antibody - BSA and Azide free (ab279642)