Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)
![Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)](https://www.abcam.com/ps/products/197/ab197072/Images/ab197072-261154-anti-trkb-phospho-y706-antibody-epr19399-western-blot.jpg "Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19399] to TrkB (phospho Y706)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
-
Product name
Anti-TrkB (phospho Y706) antibody [EPR19399]
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR19399] to TrkB (phospho Y706) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag; HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19399 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)All lanes : Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
-
![Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)](https://www.abcam.com/ps/products/197/ab197072/Images/ab197072-261153-anti-trkb-phospho-y706-antibody-epr19399-dot-blot.jpg)
Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)Dot blot analysis of TrkB (phospho Y706) labeled with ab197072 at 1/1000 dilution.
Lane 1: TrkB (phospho Y706) phospho peptide;
Lane 2: TrkB Non-phospho peptide;
Lane 3: TrkA (phospho Y680) phospho peptide;
Lane 4: TrkA Non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Note: ab197072 has weak cross reactivity with TrkA (phospho Y680) according to the dot blot data.
-
![Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)](https://www.abcam.com/ps/products/197/ab197072/Images/ab197072-3-benefits-of-recombinant-antibodies.png)
Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)
